Given the success of ioSkeletal Myocytes (ioSKM) and ioMotor Neurons (ioMTN), 糖心原创 has begun development of more advanced neuromuscular cultures. In this User Exchange we will showcase an established 28 day co-culture of ioSKM and ioMTN in a widely accessible culture format. We will explore both the successes and technical considerations of developing a neuromuscular co-culture, and provide insights into this fully ioCell-derived in vitro model. This culture methodology lays the groundwork for exploring neuromuscular junction (NMJ) formation and cellular processes such as neurodegeneration, synaptic development and axon guidance. Therefore, in collaboration with NETRI we have initiated ioMTN/ioSKM co-culture in the DuaLink microfluidic system. This work aims to build more physiologically relevant ioMTN and ioSKM interactions in a microelectrode array compatible system.

Human Cell Forum 2025
Session 1 Track 2 | From cells to systems: Building human iPSC-derived models of pain, neuromuscular junctions, and glial dynamics