cat no | io1061
ioGlutamatergic Neurons APP KM670/671NL/WT
Human iPSC-derived Alzheimer's disease model
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Cryopreserved human iPSC-derived cells powered by opti-ox that are ready for experiments in days
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Functional excitatory neurons engineered with the APP Swedish mutation for Alzheimer's disease research
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Disease-related phenotype demonstrated by increased amyloid beta production vs. wild-type control
Human iPSC-derived Alzheimer's disease model
Increased overall production of A饾浗38, A饾浗40 and A饾浗42 shown in ioGlutamatergic Neurons APP KM670/671NL (Swedish), as observed in Alzheimer鈥檚 disease
ioGlutamatergic Neurons APP KM670/671NL Alzheimer's disease model cells show an increase in the overall production of A饾浗38, A饾浗40 and A饾浗42 compared to the wild type, genetically matched control (A), and no change in the ratios of the A饾浗 peptides (B).
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ioGlutamatergic Neurons wild type (WT, io1001) and APP KM670/671NL Hom (CLH12, io1059S) and Het (CLE4, io1061S), were seeded at 30,000 cells/cm2 in 24-well plates and cultured for 30 days according to the user manual. Supernatant was collected at days 10, 20, and 30.
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Levels of A饾浗38, A饾浗40 and A饾浗42 peptides were quantified using the V-PLEX A饾浗 Peptide Panel 1 (6E10) Kit ().
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Concentrations of A饾浗38, A饾浗40, A饾浗42 were normalised to the calculated total number of cells per well.
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Data were obtained from two independent experiments and are shown as mean 卤 SEM. Data were analysed statistically (at days 20 and 30) using Student鈥檚 t-tests comparing each disease model to the wild type.
* p<0.05 ** p<0.01
ioGlutamatergic Neurons APP KM670/671NL het express neuron-specific markers comparably to the genetically matched control
Immunofluorescent staining on post-revival day 11 demonstrates similar homogenous expression of pan-neuronal proteins MAP2 and TUBB3 (upper panel) and glutamatergic neuron-specific transporter VGLUT2 (lower panel) in ioGlutamatergic Neurons APP KM670/671NL het clones compared to the genetically matched control. 100X magnification.
ioGlutamatergic Neurons APP KM670/671NL het form structural neuronal networks by day 11
ioGlutamatergic Neurons APP KM670/671NL het mature rapidly, show glutamatergic neuron morphology and form structural neuronal networks over 11 days, highly similar to the genetically matched control. Day 1 to 11 post thaw; 100X magnification.
ioGlutamatergic Neurons APP KM670/671NL het demonstrate gene expression of neuronal-specific and glutamatergic-specific markers following deterministic programming
Gene expression analysis demonstrates that ioGlutamatergic Neurons APP KM670/671NL het and wild-type ioGlutamatergic Neurons (WT Control) lack the expression of pluripotency markers (NANOG and OCT4) at day 11, whilst robustly expressing pan-neuronal (TUBB3 and SYP) and glutamatergic-specific (VGLUT1 and VGLUT2) markers, as well as the glutamate receptor GRIA4. Gene expression levels were assessed by RT-qPCR (data normalised to HMBS; cDNA samples of the parental human iPSC line (hiPSC) were included as reference). Data represents day 11 post-revival samples, n=2 replicates.
Disease-related APP is expressed in ioGlutamatergic Neurons APP KM670/671NL het following deterministic programming
RT-qPCR analysis demonstrates expression of the APP gene in both wild type ioGlutamatergic Neurons (WT Control) and ioGlutamatergic Neurons APP KM670/671NL het at day 11 post-revival. Data normalised to HMBS, n=2 replicates.
Industry leading seeding density
The recommended minimum seeding density is 30,000 cells/cm2, compared to up to 250,000 cells/cm2 for other similar products on the market. One small vial can plate a minimum of 0.7 x 24-well plate, 1 x 96-well plate, or 1.5 x 384-well plates. This means every vial goes further, enabling more experimental conditions and more repeats, resulting in more confidence in the data.
Vial limit exceeded
A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@糖心原创.
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